Alternative Names:

BCL-1 oncogene; BCL1; cyclin D1; CYL-1; G1/S-specific cyclin D1; PRAD1; PRAD1 oncogene

Application:

WB IHC IF/ICC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500

	Western blot analysis of Cyclin D1 phosphorylation expression in MCF7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

Phospho-Cyclin D1 (Thr286) Antibody detects endogenous levels of Cyclin D1 only when phosphorylated at Threonine 286

Immunogen:

A synthesized peptide derived from human Cyclin D1 around the phosphorylation site of Threonine 286

Description:

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.