Alternative Names:

ERK-1; ERK1; ERT2; Extracellular signal- regulated kinase 1; Insulin-stimulated MAP2 kinase; kinase ERK1; MAP kinase 1; MAPK 1; MAPK3; Microtubule- associated protein-2 kinase; Mitogen-activated protein kinase 3; MK03; MNK1; p44-ERK1; P44-ERK1; p44-MAPK;

Application:

WB IHC

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200

	Western blot analysis of ERK1/2 phosphorylation expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

Phospho-ERK1/2 (Tyr204) Antibody detects endogenous levels of ERK1/2 only when phosphorylated at Tyrosine 204

Immunogen:

A synthesized peptide derived from human ERK1/2 around the phosphorylation site of Tyrosine 204

Description:

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.