Alternative Names:

DNA nucleotidylexotransferase; DNTT; TDT; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal transferase;

Application:

WB IHC

Reactivity:

Human

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200

	Western blot analysis of TDT expression in Jurkat cell lysate.

Purification:

Affinity-chromatography

Specificity:

TDT Antibody detects endogenous levels of total TDT

Immunogen:

A synthesized peptide derived from human TDT

Description:

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.