Alternative Names:

CAF; CAF1; CAF1P150; CHAF1A; DCAF1; hp15; P150;

Application:

WB IHC ICC/IF IP FC

Reactivity:

Human

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:500

	Western blot analysis of p150 CAF1 expression in K562 cell lysate.

Purification:

Affinity-chromatography

Specificity:

p150 CAF1 Antibody detects endogenous levels of total p150 CAF1

Immunogen:

A synthesized peptide derived from human p150 CAF1

Description:

Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.