Alternative Names:

AOS1; HSPC140; Sae1; SUA1; UBLE1A;

Application:

WB ICC/IF IP FC

Reactivity:

Human Rat

Dilution:

WB 1:1000~1:5000 ICC/IF 1:50~1:200 IP 1:50 FC 1:100

	Western blot analysis of SAE1 expression in Jurkat cell lysate.

Purification:

Affinity-chromatography

Specificity:

SAE1 Antibody detects endogenous levels of total SAE1

Immunogen:

A synthesized peptide derived from human SAE1

Description:

The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.