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pET-47b(+)
| 质粒类型: | 大肠杆菌表达载体 |
|---|---|
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5203 bp (查看载体序列) |
| 5' 测序引物: | T7 |
| 5' 测序引物序列: | T7: 5'-TAATACGACTCACTATAGGG-3' |
| 3' 测序引物: | T7t |
| 3' 测序引物序列: | T7t: 5'-TGCTAGTTATTGCTCAGCGG-3' |
| 载体标签: | N-His, C-S, C-Thrombin |
| 载体抗性: | Kanamycin (卡那霉素) |
| 备注: |
Contains HRV 3C Protease cleavage site for fusion tag removal a low temperatures; Cterm thrombin cleavage site
|
The pET-47b(+) plasmid features the ability to express fusion proteins with an N-terminal His•Tag® coding sequence that is cleavable with the human rhinovirus (HRV) 3C protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the HRV 3C protease cleavage site (LeuGluValLeuPheGlnGlyPro), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal S•Tag™ coding sequence is compatible with purification, detection, and quantification.
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