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pGEX-5X-1
	
	 
					There are a range of Gluthatione Sepharose™ prepacked column and bulk media products available to purify GST Fusion proteins. For manual purification of sample volumes up to 600 µl use GST SpinTrap™ microspin columns or GST MultiTrap™ 4B 96-well plates. For sample volumes between 600 µl and 10 ml use GST GraviTrap™ gravity flow column. Where sample volumes are above 10 ml, use LabMate™ reservoir together with GST GraviTrap. All formats described can be used for preparation of samples in parallel. In addition GST HiTrap™ 1 and 5 ml columns and GST HiPrep™ FF 16/10 column are available for purification in a chromatography system such as the ÄKTA™ design system. Alternatively, Gluthatione Sepharose bulk media are available from 10 ml up to 500 ml. A GST Bulk Kit is also available combining 10 ml Gluthatione Sepharose 4B bulk medium and 5 empty gravity flow columns with required buffers. For simplified buffer preparation use the GST Buffer Kit. Ordering information for all associated products is listed below.
				
					
						
			
							 
						
								质粒类型:
							 
							
								大肠杆菌表达载体
							 
						
							 
						
								表达水平:
							 
							
								高拷贝
							 
						
							 
						
								启动子:
							 
							
								Tac
							 
						
							 
						
								克隆方法:
							 
							
								多克隆位点,限制性内切酶
							 
						
							 
						
								载体大小:
							 
							
								4972 bp (查看载体序列)
							 
						
							 
						
								5' 测序引物及序列:
							 
							
								pGEX5':  GGGCTGGCAAGCCACGTTTGGTG
							 
						
							 
						
								3' 测序引物及序列:
							 
							
								pGEX3': CCGGGAGCTGCATGTGTCAGAGG
							 
						
							 
						
								载体标签:
							 
							
								N-GST
							 
						
							 
						
								载体抗性:
							 
							
								Ampicillin (氨苄青霉素)
							 
						
							 
					
				
								备注:
							 
							
								 
						
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