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pGIPZ empty
Product Introduction
pGIPZ empty
| 质粒类型: | RNAi载体 |
|---|---|
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 11688 bp (查看载体序列) |
| 载体抗性: | Ampicillin (氨苄青霉素) 100µg/ml , Zeocin(博莱霉素) 25µg/ml |
| 筛选标记: | Puromycin (嘌呤霉素) |
| 备注: | 应与二代包装质粒psPAX2一同使用 |
Features of the pGIPZ™ lentiviral vector (Figure 2-3, Table 1) that make it a versatile tool for RNAi studies include:
- Ability to perform transfections or transductions using the replication incompetent lentivirus (Shimada, et al. 1995)
- TurboGFP and shRNAmir are part of a bicistronic transcript allowing the visual marking of shRNAmir expressing cells
- Amenable to in vitro and in vivo applications
- Puromycin drug resistance marker for selecting stable cell lines
- Molecular barcodes enable multiplexed screening in pools
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