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pMIB/v5-His B
Product Introduction
pMIB/v5-His B
| 质粒类型: | 昆虫细胞表达载体 |
|---|---|
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | OpIE2 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 3600bp (查看载体序列) |
| 5' 测序引物及序列: | OpIE2-F: 5'd[CGCAACGATCTGGTAAACAC]3' |
| 载体标签: | HBM (Nterm), V5 (Cterm), His (Cterm) |
| 载体抗性: | Ampicillin (氨苄青霉素) |
| 筛选标记: | Blasticidin |
| 备注: | 在昆虫细胞中能够稳定表达，HBM标签可实现目的蛋白的分泌表达 |
载体描述
pMIB/V5-His is a 3.6 kb vector designed for constitutive expression and secretion of recombinant proteins from Sf9, Sf21, and High Five™ insect cells. Secretion of proteins into serum-free medium simplifies purification, making it easier to harvest protein from cultured cells. pMIB/V5-His has several features to facilitate expression of recombinant proteins in insect cells including:
- The OpIE2 promoter for constitutive expression
- The honeybee melittin (HBM) secretion signal to allow secreted expression of your protein
- The Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks
- C-terminal V5-epitope and polyhistidine tag for detection with Invitrogens Anti-V5 antibody and purification with nickel-chelating resin
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