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pRSFDuet-1
| 质粒类型: | 大肠杆菌表达载体（双启动子） |
|---|---|
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 3829 bp (查看载体序列) |
| 5' 测序引物序列: | T7: 5'-TAATACGACTCACTATAGGG-3'; |
| 3' 测序引物序列: | T7t: 5'-TGCTAGTTATTGCTCAGCGG-3' |
| 载体标签: | N-His, C-S |
| 载体抗性: | Kanamycin (卡那霉素) |
| 备注: |
pRSFDuet-1 carries the RSF1030 replicon, lacI gene and kanamycin resistance gene.
|
pRSFDuet-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs). The vector also carries the RSF1030-derived RSF replicon, lacI gene and kanamycin resistance gene (KnR). This vector can be used in combination with pACYCDuet™-1 (Cat. No. 71147-3), pCDFDuet™-1 (Cat. Np. 71340-3), and pETDuet™-1 (Cat. No. 71146-3) in an appropriate host strain for the coexpression of 4 to 8 target proteins. ORFs inserted into MCS1 can be sequenced using the ACYCDuetUP1 Primer (Cat. No. 71178-3) and DuetDOWN1 Primer (Cat. No. 71179-3). ORFs inserted into MCS2 can be sequenced using the DuetUP2 Primer (Cat. No. 71180-3) and T7 Terminator Primer (Cat. No. 69337-3). Unique sites are shown on the circle map.
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