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pSecTag B
别名: | pSecTagB |
---|---|
质粒类型: | 哺乳动物表达载体 |
启动子: | CMV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 5170 bp (查看载体序列) |
载体标签: | IgK Leader Sequence, c-Myc Epitope Tag, His Tag (6x) |
载体抗性: | Ampicillin (氨苄青霉素) |
筛选标记: | 博莱霉素(Zeocin) |
pSecTag2 and pSecTag2/Hygro are mammalian expression vectors designed for the secretion, purification, and detection of fusion proteins. Each vector has a large multiple cloning site in three reading frames to simplify cloning in frame with the N-terminal secretion signal. The vectors (Figure 1) offer the following features:
- Secretion signal from the V-J2-C region of the mouse Ig kappa-chain for efficient secretion of recombinant proteins (Figure 2)
- Cytomegalovirus (CMV) promoter for high-level constitutive expression
- C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term) Antibody
- C-terminal c-myc epitope for detection with an Anti-myc Antibody
- Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence to enhance mRNA stability
- SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (e.g., COS-1, COS-7)
The pSecTag2 vectors carry the Zeocin™ resistance gene for cost-effective selection in mammalian cells. Zeocin™ selection can also be used in E. coli.The pSecTag2/Hygro vectors have the Hygromycin B resistance gene for selection of stable mammalian cell lines.
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