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pRNA-H1.1/Adeno
质粒类型: | RNAi载体 |
---|---|
启动子: | H1 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 8331 bp (查看载体序列) |
5' 测序引物及序列: | pRNA-H1 Forward (TAATACGACTCACTATAGGG) |
3' 测序引物及序列: | pShuttle-H1.1 Reverse (CAAAACTACATAAGACCCCCAC) |
载体标签: | cGFP |
载体抗性: | Kanamycin (卡那霉素) |
pRNAT-H1.1/Adeno is an adenoviral siRNA shuttle vector. It is compatible with the Stratagene AdEasy Adenoviral Vector System. The vector is designed for transfection in mammalian cells. An H1 promoter is used to drive siRNA expression. The vector contains a cGFP marker under the control of cytomegalovirus (CMV) promoter*, which can be used to track transfection efficiency. The vector also contains a kanamycin resistance gene for transformant selection. LITR and RITR regions are available in this vector for recombinant viral DNA replication.
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