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pRNATin-H1.2/Hygro
| 质粒类型: | RNAi载体 |
|---|---|
| 启动子: | H1.2 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6296bp (查看载体序列) |
| 5' 测序引物及序列: | pRNA-H1 Forward (TAATACGACTCACTATAGGG) |
| 3' 测序引物及序列: | pRNA Reverse (TAGAAGGCACAGTCGAGG) |
| 载体标签: | GFP |
| 载体抗性: | Ampicillin (氨苄青霉素) |
| 筛选标记: | Hygromycin |
pRNATin-H1.2/Hygro is a GenScript inducible siRNA expression vector. The H1.2 promoter is an engineered inducible promoter* containing a tetracycline operator (TetO1). The Tetracycline operator itself has no effect on expression. When the tetracycline repressor (TetR) is present, it effectively binds the TetO1 and blocks the transcription. In the presence of tetracycline or doxycycline, the inducer binds TetR and causes the TetR protein to release the TetO1 site, and derepressing the transcription from the H1 promoter.
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