NF-κB luciferase reporter plasmid （NF-κB-Luc荧光素酶报告基因质粒）

产品信息

产品描述

NF-κB-Luc荧光素酶报告基因质粒（NF-κB luciferase reporter plasmid）是钰博生物自主研发的用于检测NF-κB转录活性水平为目的的报告基因。NF-κB基因在炎症反应、免疫应答和抑制细胞凋亡等方面有着重要的作用。

NF-κB荧光素酶报告基因（报告基因质粒）主要应用于NF-κB信号通路的研究和基于NF-κB信号通路的药物研究以及癌细胞中NF-κB的调控机制的研究。

pGMNF-ΚB-Lu是钰博生物改造后的哺乳动物真核表达载体，在其多克隆位点插入了多个NF-κB结合位点，可以高灵敏度地检测NF-κB的激活水平。同时，对载体中预测出的其它转录因子以外的结合位点进行了适当的突变，增加了质粒的转录因子结合特异性。另外，由于质粒体积减小，使得NF-κB报告基因质粒更易于转染。

质粒图谱

质粒元件信息

运输与保存方法

冰袋运输。-20 ºC保存。

使用说明

1）pGMNF-Κb-Lu可以采用常规转染方法转染哺乳动物细胞。用荧光素酶检测试剂盒或双荧光素酶检测试剂盒进行检测。

2）TNF-α、IL-1β和LPS等是常见的NF-κB激活剂，可作为NF-κB报告基因的阳性对照。

注意事项

1）本质粒未经钰博生物允许不得用于任何商业用途，也不得移交给订货人实验室以外的任何人或单位。

2）为了您的健康，实验操作时请穿实验服和戴一次性手套。

参考文献

[1] Chang CT, et al. Comprehensive assessment of host responses to ionizing radiation by nuclear factor-κB bioluminescence imaging-guided transcriptomic analysis. PLoS One. 6(8):e23682(2011).

[2] Xu J, et al. Transcription of promoter from the human APRIL gene regulated by Sp1 and NF-kB. Neoplasma. 59(3):341-7(2012).

[3] Barham W, et al. Intraductal injection of LPS as a mouse model of mastitis: signaling visualized via an NF-κB reporter transgenic. J Vis Exp. Sep 4(67):e4030 (2012).

[4] Salerno KM, et al. TRAF family member-associated NF-kappa B activator (TANK) expression increases in injured sensory neurons and is transcriptionally regulated by Sox11. Neuroscience. 11.034 (2012).

[5] Nagasaki H, et al. Real-time monitoring of inflammation status in 3T3-L1 adipocytes possessing a secretory Gaussia luciferase gene under the control of nuclear factor-kappa B response element. BiochemBiophys Res Commun. 420(3):623-7(2012).

[6] Wang DJ, et al. Regulation of the let-7a-3 promoter by NF-κB. PLoS One. 7(2):e31240 (2012).

[7] Tran TA, et al. Lipopolysaccharide and tumor necrosis factor regulate Parkin expression via nuclear factor-kappa B. PLoS One. 6(8):e23660 (2011).

[8] Robbins D, et al. Imaging NF-κB signaling in mice for screening anticancer drugs. Methods Mol Biol. 716: 169-77 (2011).

[9] Dvorák Z, et al. Differential effects of selected natural compounds with anti-inflammatory activity on the glucocorticoid receptor and NF-kappaB in HeLa cells. ChemBiol Interact. 159(2):117-28 (2006).

[10] Ozeki M, et al. Response of cyclin B1 to ionizing radiation: regulation by NF-kappaB and mitochondrial antioxidant enzyme MnSOD. Anticancer Res. 24(5A):2657-63 (2004).