STAT3 luciferase reporter plasmid （STAT3-Luc荧光素酶报告基因质粒）

产品信息

产品描述

STAT3-Luc荧光素酶报告基因质粒（STAT3 luciferase reporter plasmid）是钰博生物自主研发的用于检测STAT3转录活性水平为目的的报告基因。STAT3（signal transducers and activators of transduction-3）报告基因与多种恶性肿瘤的发生、发展和侵袭转移关系密切。

STAT3报告基因主要用于监控细胞内的STAT3转录活性、肿瘤治疗药物的研发以及基因过表达和RNAi的表型分析等。

pGMSTAT3-Lu是钰博生物改造后的哺乳动物真核表达载体，在其多克隆位点插入了多个STAT3结合位点，可以高灵敏度地检测STAT3的激活水平。同时，对载体中预测出的其它转录因子以外的结合位点进行了适当的突变，增加了质粒的转录因子结合特异性。由于质粒体积减小，使得STAT3报告基因更易于转染。

质粒图谱

质粒元件信息

运输与保存方法

冰袋运输。-20 ºC保存。

注意事项

1）本质粒未经钰博生物允许不得用于任何商业用途，也不得移交给订货人实验室以外的任何人或单位。

2）为了您的健康，实验操作时请穿实验服和带一次性手套。

使用说明

pGMSTAT3-Lu可以采用常规转染方法转染哺乳动物细胞。用荧光素酶检测试剂盒或双荧光素酶检测试剂盒进行检测。

参考文献

[1] Kang Y, et al. The radio-sensitizing effect of xanthohumol is mediated by STAT3 and EGFR suppression in doxorubicin-resistant MCF-7 human breast cancer cells. Biochim Biophys Acta. 1830(3):2638-48 (2013).

[2] Teng J, et al. HBx-dependent activation of Twist mediates STAT3 Control of epithelium-mesenchymal transition of liver cells. J Cell Biochem. 114(5):1097-104 (2013).

[3] Seo HS, et al. Apigenin induces apoptosis via extrinsic pathway, inducing p53 and inhibiting STAT3 and NFκB signaling in HER2-overexpressing breast cancer cells. Mol Cell Biochem. 366(1-2):319-34 (2012).

[4] Han L, et al. MicroRNA-21 expression is regulated by β-catenin/STAT3 pathway and promotes glioma cell invasion by direct targeting RECK. CNS NeurosciTher. 18(7):573-83 (2012).

[5] Kwon MJ, et al. Protein kinase C-θ promotes Th17 differentiation via upregulation of Stat3. J Immunol. 188(12):5887-97 (2012).

[6] Lim EJ, et al. Methylsulfonylmethane suppresses breast cancer growth by down-regulating STAT3 and STAT5b pathways. PLoS One. 7(4):e33361 (2012).

[7] Hahm ER, et al. Sulforaphane inhibits constitutive and interleukin-6-induced activation of signal transducer and activator of transcription 3 in prostate cancer cells. Cancer Prev Res (Phila). 3(4):484-94 (2010).

[8] Shin DS, et al. Cryptotanshinone inhibits constitutive signal transducer and activator of transcription 3 function through blocking the dimerization in DU145 prostate cancer cells. Cancer Res. 69(1):193-202 (2009).