Recombinant Rat Interleukin-7

Accession P56478

GeneID 25647

Source Escherichia coli.

Molecular Weight Approximately 14. 9 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids.

Quantity 2µg/10µg/1mg

AA Sequence DCHIKDKDGK AFGSVLMISI NQLDKMTGTD SDCPNNEPNF FKKHLCDDTK EAAFLNRAAR KLRQFLKMNI SEEFNDHLLR VSDGTQTLVN CTSKEEKTIK EQKKNDPCFL KRLLREIKTC WNKILKGSI

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine 2E8 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

Endotoxin Less than 1 EU/µg of rRtIL-7 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

SDS-PAGE

Reference 1. Goodwin RG, Lupton S, Schmierer A, et al. 1989. Proc Natl Acad Sci U S A. 86:302-6.

2. Sutherland GR, Baker E, Fernandez KE, et al. 1989. Hum Genet. 82:371-2.

3. Lupton SD, Gimpel S, Jerzy R, et al. 1990. J Immunol. 144:3592-601.

4. Noguchi M, Nakamura Y, Russell SM, et al. 1993. Science. 262:1877-80.

5. Fry TJ, Mackall CL. 2002. Blood. 99:3892-904.

6. Fry TJ, Mackall CL. 2002. J Hematother Stem Cell Res. 11:803-7.

Background Interleukin-7 (IL-7) is encoded by the IL7 gene and secreted by stromal cells in the red marrow and thymus. It binds to the IL-7 receptor, a heterodimer consisting of IL-7 receptor alpha and IL-2 receptor gamma chain. IL-7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells and also stimulates proliferation of B cells, T cells and NK cells. Furthermore, IL-7 as an immunotherapy agent has been examined in many human clinical trials for various malignancies and during HIV infection. Rat IL-7 contains 129 amino acid residues and has three disulfide bonds. In addition, it has approximately 57 % and 88 % amino acid sequence identity with human and murine IL-7.