Recombinant Human Melanoma Inhibitor Activity Protein 2

Accession Q96PC5

GeneID 117153

Source Escherichia coli.

Molecular Weight Approximately 11.4 kDa, a single non-glycosylated polypeptide chain containing 100 amino acids.

Quantity 5µg/20µg/1mg

AA Sequence LESTKLLADL KKCGDLECEA LINRVSAMRD YRGPDCRYLN FTKGEEISVY VKLAGEREDL WAGSKGKEFG YFPRDAVQIE EVFISEEIQM STKESDFLCL

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Data Not Available.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Endotoxin Less than 1 EU/µg of rHuMIA2 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

SDS-PAGE

Reference 1. Hellerbrand C, Amann T, Schlegel J, et al. 2008. Gut, 57: 243-51.

2. Bosserhoff AK, Moser M, Scholmerich J, et al. 2003. J Biol Chem, 278: 15225-31.

3. Pitman JL, Bonnet DJ, Curtiss LK, et al. 2011. J Lipid Res, 52: 1775-86.

Background Melanoma Inhibitor Activity Protein 2 (MIA-2) encoded by MIA2 gene in humans, is a secreted cytokine that is highly expressed in liver and weakly in testis. The patients with severe fibrosis or inflammation and chronic hepatitis have higher level of MIA2 than other. Levels of MIA2 may severe as a clinically utility marker for diagnosis of hepatic disease activity and severity. The MIA2 is a member of the MIA/OTOR family, which also includes MIA, OTOR, and TANGO, and they share a Src homology-3 (SH3)-like domain.