Recombinant Human Liver-Expressed Chemokine/CCL16

Synonyms HCC-4, LCC-1, LMC, MTN-1, NCC-4

Accession O15467

GeneID 6360

Source Escherichia coli.

Molecular Weight Approximately 11.2 kDa, a single non-glycosylated polypeptide chain containing 97 amino acids.

Quantity 5µg/20µg/1mg

AA Sequence QPKVPEWVNT PSTCCLKYYE KVLPRRLVVG YRKALNCHLP AIIFVTKRNR EVCTNPNDDW VQEYIKDPNL PLLPTRNLST VKIITAKNGQ PQLLNSQ

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Endotoxin Less than 1 EU/μg of rHuLEC/CCL16 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Reference 1. Hedrick JA, Helms A, Vicari A, et al. 1998. Blood, 91: 4242-7.

2. Nomiyama H, Fukuda S, Iio M, et al. 1999. J Interferon Cytokine Res, 19: 227-34.

3. Youn BS, Zhang S, Broxmeyer HE, et al. 1998. Biochem Biophys Res Commun, 247: 217-22.

4. Kim IS, Jang SW, Sung HJ, et al. 2005. FEBS Lett, 579: 6044-8.

5. Francica G, Petrolati A, Di Stasio E, et al. 2012. Acta Radiol, 53: 394-400.

Background Human CCL16, also called Liver-expressed chemokine (LEC), Monotactin-1 (MTN-1), IL-10-inducible chemokine and so on, is expressed by the CCL16 gene located on the chromosome 17 in humans. The gene encodes a 120 a.a. residue precursor protein with a 23 a.a. residue predicted signal peptide that is cleaved to generate a 97 a.a. residue mature protein. The protein is secreted by the liver, thymus, spleen cells and showing chemotactic activity for lymphocytes and monocytes but it is distantly related to other CC chemokines, exhibiting less than 30 % sequence identity. CCL16 is highly induced by IL-10, IFN-γ and bacterial lipopolysaccharide in monmcytes and signal through CCR1, CCR2, CCR5, and CCR8.