Recombinant Human Cyclophilin D

Synonyms PPIF, Cyclophilin F, CyP-M, Rotamase F

Accession P30405

GeneID 10105

Unigene Hs.381072

Source Escherichia coli.

Molecular Weight Approximately 18.9 kDa, a single non-glycosylated polypeptide chain containing 178 amino acids.

AA Sequence CSKGSGDPSS SSSSGNPLVY LDVDANGKPL GRVVLELKAD VVPKTAENFR ALCTGEKGFG YKGSTFHRVI PSFMCQAGDF TNHNGTGGKS IYGSRFPDEN FTLKHVGPGV LSMANAGPNT NGSQFFICTI KTDWLDGKHV VFGHVKEGMD VVKKIESFGS KSGRTSKKIV ITDCGQLS

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity Data Not Available.

Physical Appearance Sterile colorless liquid.

Formulation Supplied as a 0.2 μm filtered solution in PBS, pH7.4, with 1 mM DTT, 10 % glycerol.

Endotoxin Less than 0.1 EU/μg of rHuCyP-D as determined by LAL method.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 6 months from date of receipt, -20 to -70 °C as supplied.

- 3 months, -20 to -70 °C under sterile conditions after opening.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Background CyP-D is a part of the peptidyl-prolyl cis-trans isomerase (PPIase) family. CyP-D accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. CyP-D is key component of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.