Recombinant Human Cardiac muscle troponin T

Accession P45379

GeneID 7139

Source Escherichia coli.

Molecular Weight Approximately 35.8 kDa, a single non-glycosylated polypeptide chain containing 297 amino acids.

AA Sequence SDIEEVVEEY EEEEQEEAAV EEEEDWREDE DEQEEAAEED AEAEAETEET RAEEDEEEEE AKEAEDGPME ESKPKPRSFM PNLVPPKIPD GERVDFDDIH RKRMEKDLNE LQALIEAHFE NRKKEEEELV SLKDRIERRR AERAEQQRIR NEREKERQNR LAEERARREE EENRRKAEDE ARKKKALSNM MHFGGYIQKQ AQTERKSGKR QTEREKKKKI LAERRKVLAI DHLNEDQLRE KAKELWQSIY NLEAEKFDLQ EKFKQQKYEI NVLRNRINDN QKVSKTRGKA KVTGRWK

Purity > 98 % by SDS-PAGE and HPLC analyses.

Biological Activity Data not available.

Physical Appearance Sterile colorless liquid.

Formulation Supplied as a 0.2 μm filtered solution in 10 mM Tris-HCl, pH8.0, 250 mM NaCl, with 50% glycerol.

Endotoxin Less than 1 EU/µg of rHucTnT as determined by LAL method.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 6 months from date of receipt, -20 to -70 °C as supplied.

- 3 months, -20 to -70 °C under sterile conditions after opening.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Background Troponin T is one of three troponin isoforms found in the tropomyosin-troponin complex. This complex is responsible for the calcium sensitivity of the contractile apparatus in the muscle. Cardiac Troponin T is used as a biological marker for cardiomyocytes and its level in serum is frequently used as an indicator of myocardial cell injury.