Recombinant Bungia malayi Asparagine tRNA Synthetase

Source: Escherichia coli.

Molecular Weight: Approximately 64.5 kDa, a single non-glycosylated polypeptide chain containing 568 amino acids.

AA Sequence: MGSSHHHHHH SSGLVPRGSH MTVYICPETG DDGNDGSELK PLRTLYQAMI ITKSSKGDFL IRTKKDGKQV WEAASKTALK KSWKRYEQEM LKNEKVAAKM LEKDATEVGV KAALEEAKKV QIELDTSLSY ITGVKIRDLV KHRNERVCIK GWIHRMRRQG KSLMFFILRD GTGFLQVLLM DKLCQTYDAL TVNTECTVEI YGAIKEVPEG KEAPNGHELI ADFWKIIGNA PSGGIDNVLN EEASVDKMLD NRHLVIRGEN AAALLRLRAA ATRAMREHFY NAGYVEVAPP TLVQTQVEGG STLFNLDYFG EQSFLTQSSQ LYLETCIPTL GDVFLHCSVL QGGKISHSST LAEYAHVEAE CPFITLDDLM EKIEELVCDT VDRLLADEEA KKLLEHINPK FQPPERPFLR MEYKDAIKWL QEHNVENEFG NTFTYGEDIA EAAERFMTDT INKPILLNRF PSEIKAFYMQ RDAKDNTLTE SVDLLMPGVG EIVGGSMRIW KFDELSKAFK NVEIDPKPYY WYLDQRLYGT CPHGGYGLGL ERFICWLTNT NHIRDVCLYP RFVGRCVP

Purity: > 95 % by SDS-PAGE.

Biological Activity: Data Not Available.

Physical Appearance: Sterile colorless liquid.

Formulation: Supplied as a 0.2 μm filtered solution in 20 mM HEPES, pH7.4, 100 mM NaCl, 5 mM MgCl2, 5 mM β-Mercaptoethanol, 10 % Glycerol.

Endotoxin: Less than 1 EU/μg of rBmAsnRS as determined by LAL method.

Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 6 months from date of receipt, -20 to -70 °C as supplied.

- 3 months, -20 to -70 °C under sterile conditions after opening.

Usage: This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Background The Bungia malayi asparagine-tRNA ligase (also known as asparaginyl-tRNA synthetase/AsnRs) catalyses chemical reaction:

ATP + L-asparagine + tRNAAsn ↔AMP + diphosphate + L-asparaginyl-tRNAAsn.

AsnRS is an alpha2 dimer that belongs to class IIb, to be specific those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. AsnRS is considered an excellent antifilarial target to cure for Lymphatic filariasis.