Recombinant Human Neutrophil Activating Protein-2/CXCL7

Synonyms CXCL7, LDGF, MDGF, Small-inducible cytokine B7

Accession P02775

GeneID 5473

Source Escherichia coli.

Molecular Weight Approximately 7.6 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.

Quantity 2µg/10µg/1mg

AA Sequence AELRCMCIKT TSGIHPKNIQ SLEVIGKGTH CNQVEVIATL KDGRKICLDP DAPRIKKIVQ KKLAGDESAD

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 1.0-10.0 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.

Endotoxin Less than 1 EU/μg of rHuNAP-2/CXCL7 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Reference 1. Hristov M, Zernecke A, Bidzhekov K, et al. 2007. Circ Res. 100:590-7.

2. Majumdar S, Gonder D, Koutsis B, et al. 1991. J Biol Chem. 266:5785-9.

3. Krijgsveld J, Zaat SA, Meeldijk J, et al. 2000. J Biol Chem. 275:20374-81.

4. Piccardoni P, Evangelista V, Piccoli A, et al. 1996. Thromb Haemost. 76:780-5.

Background Neutrophil activating protein-2 also named CXCL7 is an isoform of Beta-Thromboglobulin or Pro-Platelet basic protein. It belongs to the CXC chemokine family and is released in large amounts from platelets following their activation. CXCL7 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. Recombinant human CXCL7 contains 70 amino acids which is a single non-glycosylated polypeptide chain. In addition, The human CXCL7 shares 53 % and 58 % a.a. sequence identity with mouse and rat CXCL7.