Recombinant Human Monokine Induced by Interferon-gamma/CXCL9

Synonyms Monokine-like Protein

Accession Q07325

GeneID 4283

Source Escherichia coli.

Molecular Weight Approximately 11.7 kDa, a single non-glycosylated polypeptide chain containing 103 amino acids.

Quantity 5µg/20µg/1mg

AA Sequence TPVVRKGRCS CISTNQGTIH LQSLKDLKQF APSPSCEKIE IIATLKNGVQ TCLNPDSADV KELIKKWEKQ VSQKKKQKNG KKHQKKKVLK VRKSQRSRQK KTT

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood T-lymphocytes is in a concentration range of 10-100 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.

Endotoxin Less than 1 EU/μg of rHuMIG/CXCL9 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

SDS-PAGE

Reference 1. Lee HH, Farber JM. 1996. Cytogenet Cell Genet. 74:255-8.

2. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42.

3. Tensen CP, Flier J, Van Der Raaij-Helmer EM, et al. 1999. J Invest Dermatol. 112:716-22.

Background CXCL9 is a T-cell chemoattractant induced by IFN-γ belonging to the CXC chemokine family and it is also known as Monokine induced by gamma interferon (MIG). CXCL9 is closely related to two other CXC chemokines called CXCL10 and CXCL11 and they all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3. CXCL9 is a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response and chemotactic for activated T-cells. Recombinant human CXCL9 contains 103 amino acids which is a single non-glycosylated polypeptide chain. The human CXCL9 shares 75 % and 67 % a.a. sequence identity with mouse and rat CXCL9.