Recombinant Murine Glia Maturation Factor beta

Synonyms GMFB

Accession Q9CQI3

GeneID 63985

Source Escherichia coli.

Molecular Weight Approximately 16.6 kDa, a single non-glycosylated polypeptide chain containing 141 amino acid residues.

Quantity 2µg/10µg/1mg

AA Sequence SESLVVCDVA EDLVEKLRKF RFRKETHNAA IIMKIDKDER LVVLDEELEG VSPDELKDEL PERQPRFIVY SYKYQHDDGR VSYPLCFIFS SPVGCKPEQQ MMYAGSKNKL VQTAELTKVF EIRNTEDLTE EWLREKLGFF H

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Data not available.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Endotoxin Less than 1 EU/μg of rMuGMF-β as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Reference 1. Gandhi M, Smith BA, Bovellan M, et al. 2010. Curr Biol, 20: 861-7.

2. Roman AandTombarkiewicz B. 2009. Bioelectromagnetics, 30: 21-8.

3. Ohnishi T, Arita N, Hayakawa T, et al. 1993. Biochem Biophys Res Commun, 193: 518-25.

4. Nakano K, Kuwayama H, Kawasaki M, et al. 2010. Cytoskeleton (Hoboken), 67: 373-82.

Background The glia maturation factor beta belongs to the actin-binding proteins ADF family, GMF subfamily. It contains an ADF-H domain, but the research of crystallography and NMR reveals that there are structures different between human and mouse ADF-H domain. GMF-β is involved in the differentiation, maintenance, and regeneration of the nervous system. It also inhibition of proliferation of tumor cells.