Recombinant Murine Midkine

Synonyms Retinoic Acid-induced Differentiation Factor

Accession P12025

GeneID 17242

Source Escherichia coli.

Molecular Weight Approximately 13.2 kDa, a single non-glycosylated polypeptide chain containing 120 amino acids.

Quantity 5µg/20µg/1mg

AA Sequence VAKKKEKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRVHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK TKAKKGKGKD

Purity > 96 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-100 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH7.4.

Endotoxin Less than 1 EU/μg of rMuMidkine as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

SDS-PAGE

Reference 1. Kaname T, Kuwano A, Murano I, et al. 1993. Genomics. 17:514-5.

2. Ikematsu S, Yano A, Aridome K, et al. 2000. Br J Cancer. 83:701-6.

3. Muramatsu T. 2002. J Biochem. 132:359-71.

Background Midkine, also named MK, MK1, NEGF 2, is belonging to the neurotrophic and developmentally-regulated heparin-binding molecules family. It is encoded by the MDK gene. The Midkine protein includes five intrachain disulfide bonds which hold two domains and there are three antiparallel beta-sheets in each domain. A chondroitin sulfate proteoglycan, protein-tyrosine phosphatase zeta (PTPzeta), is a receptor for MK. MK promotes the growth, survival, and migration of various cells, and plays roles in neurogenesis and epithelial mesenchymal interactions during organogenesis. The predicted molecular weight is approximately 13.3 kDa, based on a mature peptide length of 118 amino acid residues in the mouse and 121 amino acid residues in the human. Across species, MK shows 87 % identity between the human and murine proteins.