Recombinant Human Bone Morphogenetic Protein 7

Synonyms OP-1

Accession P18075

GeneID 655

Source Escherichia coli.

Molecular Weight Approximately 15.7 kDa, a monomeric, non-glycosylated polypeptide chain containing 139 amino acids.

Quantity 2µg/10µg/1mg

AA Sequence STGSKQRSQN RSKTPKNQEA LRMANVAENS SSDQRQACKK HELYVSFRDL GWQDWIIAPE GYAAYYCEGE CAFPLNSYMN ATNHAIVQTL VHFINPETVP KPCCAPTQLN AISVLYFDDS SNVILKKYRN MVVRACGCH

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity Data is not available.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA.

Endotoxin Less than 1 EU/μg of rHuBMP-7 as determined by LAL method.

Applications 1. Molecular standard (Western, ELISA) in studying secreted BMP-7;

2. Preparing antibodies for BMP-7 monomer;

3. Molecule standard in detecting secreted BMP-7 in reduced SDS-PAGE.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

SDS-PAGE

Reference 1. Zeisberg M, Hanai J, Sugimoto H, et al. 2003. Nat Med, 9: 964-8.

2. Phillips FM, Turner AS, Seim HB, 3rd, et al. 2006. Spine J, 6: 500-6.

3. Veerasamy M, Nguyen TQ, Motazed R, et al. 2009. Am J Physiol Renal Physiol, 297: F1238-48.

4. Pauly S, Klatte F, Strobel C, et al. 2012. J Shoulder Elbow Surg, 21: 464-73.

Background Bone Morphogenetic Protein 7 is one of the BMPs, some of which belong to the TGF-beta superfamily (BMP2-7). There are more than thirteen BMPs have been discovered nowadays and they are involved in inducing cartilage and bone formation. BMP-7 is mainly expressed in kidney and bladder. It is also present in developing eyes, brain and ear during embryogenesis. BMP-7 also named osteogenic protein-1 (OP-1) is a potent osteoinductive cytokine and plays role in osteoblast differentiation, SMAD1 production and renal development and repair. Human BMP-7 is synthesized with a signal sequence (29 a.a.), a propeptide (263 a.a.), and a growth factor domain (139 a.a.). The growth factor domain of human BMP-7 shares 98 % a.a. sequence identity with mouse and rat BMP-7.