Recombinant Human Neuregulin 1-beta2 EGF-like Domain

Synonyms Heregulin-beta1, HRG1

Accession Q02297

GeneID 3084

Source Escherichia coli.

Molecular Weight Approximately 7.0 kDa, a single non-glycosylated polypeptide chain containing 61 amino acids.

Quantity 10µg/50µg/1mg

AA Sequence SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKAEELY Q

Purity > 96 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104 IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.

Endotoxin Less than 1 EU/μg of rHuNRG1-β2 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

SDS-PAGE

Reference 1. Shamir A, Kwon OB, Karavanova I, et al. 2012. J Neurosci, 32: 2988-97.

2. Malek RL, Toman RE, Edsall LC, et al. 2001. J Biol Chem, 276: 5692-9.

3. Zhang Z, Prentiss L, Heitzman D, et al. 2006. J Neurosci Res, 84: 1-12.

4. Law AJ, Shannon Weickert C, Hyde TM, et al. 2004. Neuroscience, 127: 125-36.

Background Neuregulin 1 belongs to a family of structurally related polypeptide growth factors and is produced in numerous isoforms by alternative splicing, which allows it to perform a wide variety of functions. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They all have the Ig and EGF-like domain, and can bind to ErbB3 and ErbB4 receptor tyrosin kinases. This binding induces ErbB3 and ErbB4 heterodimerization with ErbB2, stimulating intrinsic kinase activity, which leads to tyrosine phosphorylation. NRG1 isoforms have functions of inducing the growth and differentiation of epithelial, neuronal, glial, and other types of cells.