Recombinant Human B cell Activating Factor Rececptor/TNFRSF13C

Synonyms BAFFR, TNFRSF13C

Accession Q96RJ3

GeneID 115650

Source Escherichia coli.

Molecular Weight Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids.

Quantity 10µg/50µg/1mg

AA Sequence MRRGPRSLRG RDAPAPTPCV PAECFDLLVR HCVACGLLRT PRPKPAGASS PAPRTALQPQ ESVGAGAGEA ALPLPG

Purity > 95 % by reduced SDS-PAGE analyses.

Biological Activity Fully biologically active when compared to standard. The ED50 as determined by its ability to block BAFF induced mouse splenocyte survival is 1.0-5.0 μg/ml in the presence of 1.0 μg/ml of rHuBAFF.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 8.0, 500 mM NaCl.

Endotoxin Less than 1 EU/μg of rHuBAFF-R as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Reference 1. Castigli E, Wilson SA, Scott S, et al. 2005. J Exp Med, 201: 35-9.

2. Nakajima K, Itoh K, Nagatani K, et al. 2007. Scand J Rheumatol, 36: 365-72.

3. Zhu XJ, Shi Y, Peng J, et al. 2009. Blood, 114: 5362-7.

4. Thibault-Espitia A, Foucher Y, Danger R, et al. 2012. Am J Transplant, 12: 2754-62.

Background B Cell Activating Factor Receptor (BAFF-R), also named tumor necrosis factor receptor superfamily member 13C, is a member of the TNFR superfamily. It is highly expressed in spleen, lymph node, and resting B cells and to some extent in activated B cells, resting CD4+ cells and peripheral blood leukocytes. BAFF receptor is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain and binds with high specificity to BAFF (TNFSF13B). It enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. BAFF receptor/BAFF signaling plays a critical role in B cell survival and maturation.