Recombinant Human soluble CD40 Ligand/TNFSF5

Synonyms TNFSF5, sCD40L, CD154, gp39, T-BAM, TNF-related Activation Protein, TRAP

Accession P29965

GeneID 959

Source Escherichia coli.

Molecular Weight Approximately 16.3 kDa, a single non-glycosylated polypeptide chain containing 149 amino acids.

Quantity 10µg/50µg/1mg

AA Sequence MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.

Endotoxin Less than 1 EU/μg of rHusCD40L as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-0.3 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Reference 1. Li R, Chen WC, Pang XQ, et al. 2011. Mol Biol Rep, 38: 5459-64.

2. Reinboldt S, Wenzel F, Rauch BH, et al. 2009. Platelets, 20: 441-4.

3. Tousoulis D, Antoniades C, Nikolopoulou A, et al. 2007. Eur J Clin Invest, 37: 623-8.

4. Varo N, Libby P, Nuzzo R, et al. 2005. Diab Vasc Dis Res, 2: 81-7.

5. Holzer G, Pfandlsteiner T, Blahovec H, et al. 2003. Wien Med Wochenschr, 153: 40-2.

Background CD40 ligand is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF family and can be cleaved into two chains: the CD40 ligand membrane form and CD40 ligand soluble form. It is primarily expressed on activated CD4+ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. It mediates a range of activities on B cells including induction of activation-associated surface antigen, entry into cell cycle, isotype switching and Ig secretion and memory generation. CD40-CD40L interaction also plays important roles in monocyte activation and dendritic cell maturation.