Recombinant Human 4-1BB Receptor/TNFRSF9

Synonyms TNFRSF9, CD137 Antigen, T-cell Antigen ILA

Accession Q07011

GeneID 3604

Source Escherichia coli.

Molecular Weight Approximately 17.7 kDa, a single non-glycosylated polypeptide chain containing 166 amino acids.

Quantity 5µg/20µg/1mg

AA Sequence ERTRSLQDPC SNCPAGTFCD NNRNQICSPC PPNSFSSAGG QRTCDICRQC KGVFRTRKEC SSTSNAECDC TPGFHCLGAG CSMCEQDCKQ GQELTKKGCK DCCFGTFNDQ KRGICRPWTN CSLDGKSVLV NGTKERDVVC GPSPADLSPG ASSVTPPAPA REPGHS

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The biological activity is determined by its inhibitory effect of IL-8 production using human peripheral blood mononuclear cells. About 90 % of inibition was seen using a concentration of 1 µg for both 4-1BB Ligand and 4-1BB Receptor.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM PB, pH 8.0, 150 mM NaCl.

Endotoxin Less than 1 EU/μg of rHu4-1BB Receptor as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

SDS-PAGE

Reference 1. Arch RHandThompson CB. 1998. Mol Cell Biol, 18: 558-65.

2. Barao I. 2012. Front Immunol, 3: 402.

3. Taraban VY, Rowley TF, O'Brien L, et al. 2002. Eur J Immunol, 32: 3617-27.

4. Marin V, Kakuda H, Dander E, et al. 2007. Exp Hematol, 35: 1388-97.

Background 4-1BB receptor, also named TNFRSF9 is a member of the TNF superfamily of receptors. It is mainly expressed on the surface of a variety of T cells, but also found in B cells, monocytes, and various transformed cell lines. 4-1BB receptor binds to 4-1BBL, and they co-stimulate activity for activated T cells. Signaling by 4-1BB Receptor has been implicated in the antigen-presentation process and generation of cytotoxic T cells. Crosslinking of 4-1BB Receptor enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice.