Recombinant Human B-Cell Maturation Antigen/TNFRSF17

Synonyms TNFRSF17, BCM, CD269

Accession Q02223

GeneID 608

Source Escherichia coli.

Molecular Weight Approximately 5.4 kDa, a single non-glycosylated polypeptide chain containing 50 amino acids.

Quantity 5µg/20µg/1mg

AA Sequence AGQCSQNEYF DSLLHACIPC QLRCSSNTPP LTCQRYCNAS VTNSVKGTNA

Purity > 98 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit APRIL-mediated proliferation of anti-IgM stimulated murine B cells is no less than 40 ng/ml, corresponding to a specific activity of > 2.5 × 104 IU/mg in the presence of 100 ng/ml human APRIL.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA.

Endotoxin Less than 1 EU/μg of rHuBCMA as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Reference 1. Kim J, Gross JA, Dillon SR, et al. 2011. Autoimmunity, 44: 69-81.

2. Sun J, Feng J, Li Y, et al. 2006. Biochem Biophys Res Commun, 346: 1158-62.

3. Pelletier M, Thompson JS, Qian F, et al. 2003. J Biol Chem, 278: 33127-33.

4. Laabi Y, Gras MP, Brouet JC, et al. 1994. Nucleic Acids Res, 22: 1147-54.

Background B-Cell Maturation Antigen (BCMA), encoded by the TNFRSF17 gene in humans. It is a member of the TNF receptor superfamily and a type III membrane protein containing one extracellular cysteine rich domain. It is expressed on mature B-cells and other B-cell lines. BCMA can binds to TNFSF13B/BLyS/BAFF and TNFSF13/APRIL, and has functions that promotes B-cell survival, plays a role in the regulation of humoral immunity, and activates NF-kappa-B and JNK BCMA.