Recombinant Murine Monocyte Chemotactic Protein-3/CCL7

Synonyms Protein FIC, Small-inducible Cytokine A7, Intercrine/chemokine MARC

Accession Q03366

GeneID 20306

Source Escherichia coli.

Molecular Weight Approximately 8.5 kDa, a single, non-glycosylated polypeptide chain containing 74 amino acids.

Quantity 2µg/10µg/1mg

AA Sequence QPDGPNASTC CYVKKQKIPK RNLKSYRRIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTP TPKP

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 100-300 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 µm filtered concentrated solution in 2× PBS, pH 7.4.

Endotoxin Less than 1 EU/µg of rMuMCP-3/CCL7 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

SDS-PAGE

Reference 1. Kulmburg PA, Huber NE, Scheer BJ, et al. 1992. J Exp Med. 176:1773-8.

2. Thirion S, Nys G, Fiten P, et al. 1994. Biochem Biophys Res Commun. 201:493-9.

Background Murine CCL7, also known as MARC, is belonging to the CC chemokine family. It is encoded by the gene CCL7 and was isolated from a mouse mast cell line after Fc epsilon RI triggering by IgE plus antigen. Sequence comparisons suggest that MARC may be the mouse homologue of the human MCP-3 gene. Similar to the human system, MARC/FIC is postulated the murine MCP-3. The MCP-3 protein family signals through CCR2 expect MCP-1 and possess cross-reacts across species. CCL7/MCP3 has chemotactic function for monocytes and eosinophils, but not for neutrophils.