YBD060Ra21 Monoclonal Antibody to Heat Shock 70kDa Protein 2 (HSPA2)

Organism Species: Rattus norvegicus (Rat)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

9th Edition (Revised in Jul, 2013)

[ PRODUCT INFORMATION ]                                                           

Immunogen: HSPA2, Rat

Clonality: Monoclonal

Host: Mouse

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 500µg/mL

UOM: 200µg Sample: Recombinant HSPA2, Rat

[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant HSPA2 (Met1~Asp633) expressed in E.coli.

USCN Accession No.: RPD060Ra01

Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below.

MGHHHHHHSG SEF-MSARGPAIGI DLGTTYSCVG VFQHG KVEII AN DQGNRTTP

SYVAFTDTER LIGDAAKNQV AMNPTNTIFD AKRLIGRKFE DATVQSDMKH WPFRVVSEGG

KPKVQVEYKG EMKTFFPEEI SSMVLTKMKE IAEAYLGGKV QSAVITVPAY FNDSQRQATK

DAGTITGLNV LRIINEPTAA AIAYGLDKKG CAGGEKNVLI FDLGGGTFDV SILTIEDGIF

EVKSTAGDTH LGGEDFDNRM VSHLAEEFKR KHKKDIGPNK RAVRRLRTAC ERAKRTLSSS

TQASIEIDSL YEGVDFYTSI TRARFEELNA DLFRGTLEPV EKALRDAKLD KGQIQEIVLV

GGSTRIPKIQ KLLQDFFNGKELNKSINPDE AVAYGAAVQA AILIGDKSEN VQDLLLLDVT

PLSLGIETAG GVMTPLIKRN TTIPTKQTQT FTTYSDNQSS VLVQVYEGER AMTKDNNLLG

KFDLTGIPPA PRGVPQIEVT FDIDANGILN VTAADKSTGK ENKITITNDK GRLSKDDIDR

MVQEAERYKS EDEANRDRVA AKNAVESYTY NIKQTVEDEK LRGKISEQDK NKILDKCQEV

INWLDRNQMA EKDEYEHKQK ELERVCNPII SKLYQGGPGG GGSSGGPTIE EVD

[ ANTIBODY SPECIFITY ]

The antibody is a mouse monoclonal antibody raised against HSPA2. It has been selected for its ability to recognize HSPA2 in immunohistochemical staining and western blotting.

[ APPLICATIONS ]

Western blotting: 1:100-400

Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.

[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ STORAGE ]

Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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