YBD776Hu01 100ug

Recombinant Protein Disulfide Isomerase A6 (PDIA6)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

10th Edition (Revised in Jan, 2014)

[ PROPERTIES ]

Residues: Leu20~Leu440

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q15084

Host: E. coli

Subcellular Location: Endoplasmic reticulum lumen.

Cell membrane. Melanosome.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 5.0

Predicted Molecular Mass: 49.9kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

TEL:4006-871-227 Web:www.ybio.net Email:shybio@126.com

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

L YSSSDDVIEL TPSNFNREVI QSDSLWLVEF YAPWCGHCQR LTPEWKKAAT ALKDVVKVGA

VDADKHHSLG GQYGVQGFPT IKIFGSNKNR PEDYQGGRTG EAIVDAALSA LRQLVKDRLG

G R S G G Y S S G K Q G R S D S S S K K D V I E LT D D S F D K N V L D S E D V W M V E F YA P W C

GHCKNLEPEW AAAASEVKEQ TKGKVKLAAV DATVNQVLAS RYGIRGFPTI KIFQKGESPV

DYDGGRTRSD IVSRALDLFS DNAPPPELLE IINEDIAKRT CEEHQLCVVA VLPHILDTGA

AGRNSYLEVL LKLADKYKKK MWGWLWTEAG AQSELETALG IGGFGYPAMA AINARKMKFA

LLKGSFSEQG INEFLRELSF GRGSTAPVGG GAFPTIVERE PWDGRDGELP VEDDIDLSDV

ELDDLGKDEL

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