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蛋白激酶 Cβ1(PKCβ1)重组蛋白
蛋白激酶 Cβ1(PKCβ1)重组蛋白

蛋白激酶 Cβ1(PKCβ1)重组蛋白 StabilityTest:Thethermalstabilityisdescribedbythelossrateofthetargetprotein.Theloss ratewasdeterminedbyacceleratedthermaldegradationtest,thatis,incubatetheproteinat37o Cfor48h,andnoobviousdegradationandprecipitationwereobserved.

Product Introduction


蛋白激酶 Cβ1(PKCβ1)重组蛋白


适用生物Oryctolagus cuniculus (Rabbit,兔)

产品应用SDS-PAGE; WB; ELISA; IP.规格50ug


分子量34.8kDa价格请咨询当地代理商纯度> 95%

来源原核表达


RecombinantProteinKinaseCBeta1(PKCb1)


FORINVITROANDRESEARCHUSEONLY,NOTFORUSEINCLINICALDIAGNOSTICPRO


CEDURES!


Organism species Oryctolagus cuniculus (Rabbit)


Product No. YBB045Rb01


Source Prokaryotic expression


Host E.coli


Purity > 95%


UOM 50ug


Predicted Molecular Mass 34.8kDa


Predicted isoelectric

n/a

point


Applications SDS-PAGE; WB; ELISA; IP.


Endotoxin Level <1.0EU per 1µg (determined by the LAL method)






Subcellular Location n/a


Residues Phe342~Phe600 (Accession # P05772) with two N-terminal Tags, His-tag and S-tag


Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosy




SEQUENCES



TEL:4006-871-227 Web:www.ybio.net Email:shybio@126.com


ThetargetproteinisfusedwithtwoN-terminalTags,His-tagandS-tag,itssequenceislistedbe low.


MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGS-FNFLMVLGKGSFGKV


MLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVM


EYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFG


MCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELF


QSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKDHAFF



USAGE



ReconstituteinsterilePBS,pH7.2-pH7.4.



STORAGEANDSTABILITY


Storage:Avoidrepeatedfreeze/thawcycles.Storeat2-8oCforonemonth.Aliquotandstoreat


-80oCfor12months.


StabilityTest:Thethermalstabilityisdescribedbythelossrateofthetargetprotein.Theloss


ratewasdeterminedbyacceleratedthermaldegradationtest,thatis,incubatetheproteinat37o Cfor48h,andnoobviousdegradationandprecipitationwereobserved.(ReferringfromChinaBi ologicalProductsStandard,whichwascalculatedbytheArrheniusequation.)Thelossofthispro teinislessthan5%withintheexpirationdateunderappropriatestoragecondition.



AbouttheMARKER(complimentary)


EffectiveSizeRange:10kDato70kDa.


Proteinbands:10kDa,14kDa,18kDa,22kDa,26kDa,33kDa,44kDaand70kDa.


Doubleintensitybands:The26kDa,18kDa,10kDabandsareatdoubleintensitytomakeloc ationandsizeapproximationofproteinsofinterestquickandeasy.


Ready-to-use:Noneedtoheat,diluteoraddreducingagentsbeforeuse.

 

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