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蛋白激酶 Cβ1(PKCβ1)重组蛋白 StabilityTest:Thethermalstabilityisdescribedbythelossrateofthetargetprotein.Theloss ratewasdeterminedbyacceleratedthermaldegradationtest,thatis,incubatetheproteinat37o Cfor48h,andnoobviousdegradationandprecipitationwereobserved.
蛋白激酶 Cβ1(PKCβ1)重组蛋白
适用生物Oryctolagus cuniculus (Rabbit,兔)
产品应用SDS-PAGE; WB; ELISA; IP.规格50ug
分子量34.8kDa价格请咨询当地代理商纯度> 95%
来源原核表达
RecombinantProteinKinaseCBeta1(PKCb1)
FORINVITROANDRESEARCHUSEONLY,NOTFORUSEINCLINICALDIAGNOSTICPRO
CEDURES!
Organism species Oryctolagus cuniculus (Rabbit)
Product No. YBB045Rb01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 34.8kDa
Predicted isoelectric
n/a
point
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Subcellular Location n/a
Residues Phe342~Phe600 (Accession # P05772) with two N-terminal Tags, His-tag and S-tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosy
SEQUENCES
TEL:4006-871-227 Web:www.ybio.net Email:shybio@126.com
ThetargetproteinisfusedwithtwoN-terminalTags,His-tagandS-tag,itssequenceislistedbe low.
MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGS-FNFLMVLGKGSFGKV
MLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVM
EYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFG
MCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELF
QSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKDHAFF
USAGE
ReconstituteinsterilePBS,pH7.2-pH7.4.
STORAGEANDSTABILITY
Storage:Avoidrepeatedfreeze/thawcycles.Storeat2-8oCforonemonth.Aliquotandstoreat
-80oCfor12months.
StabilityTest:Thethermalstabilityisdescribedbythelossrateofthetargetprotein.Theloss
ratewasdeterminedbyacceleratedthermaldegradationtest,thatis,incubatetheproteinat37o Cfor48h,andnoobviousdegradationandprecipitationwereobserved.(ReferringfromChinaBi ologicalProductsStandard,whichwascalculatedbytheArrheniusequation.)Thelossofthispro teinislessthan5%withintheexpirationdateunderappropriatestoragecondition.
AbouttheMARKER(complimentary)
EffectiveSizeRange:10kDato70kDa.
Proteinbands:10kDa,14kDa,18kDa,22kDa,26kDa,33kDa,44kDaand70kDa.
Doubleintensitybands:The26kDa,18kDa,10kDabandsareatdoubleintensitytomakeloc ationandsizeapproximationofproteinsofinterestquickandeasy.
Ready-to-use:Noneedtoheat,diluteoraddreducingagentsbeforeuse.
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