YBC285Hu71 Biotin-Linked Antibody to Anterior Gradient Protein 2 (AGR2)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

9th Edition (Revised in Jul, 2013)

[ PRODUCT INFORMATION ]

Immunogen: AGR2, Human

Clonality: Monoclonal

Conjugation: Biotin

Clone number: F5

Host: Mouse

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200µg/mL

UOM: 200µg Sample: Recombinant AGR2, Human

[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant AGR2 (Arg21~Leu175) expressed in E.coli.

Accession No.: RPC285Hu01

Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag and its sequence is listed below.

MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF-

RDTTVKPGAK KDTKDSRPKL PQTLSRGWGD QLIWTQTYEE ALYKSKTSNK PLMIIHHLDE

CPHSQALKKV FAENKEIQKL AEQFVLLNLV YETTDKHLSP DGQYVPRIMF VDPSLTVRAD

TEL:4006-871-227 Web:www.ybio.net Email:shybio@126.com

ITGRYSNRLY AYEPADTALL LDNMKKALKL LKTEL

TEL:4006-871-227 Web:www.ybio.net Email:shybio@126.com

[ ANTIBODY SPECIFITY ]

Anti AGR2 is a mouse monoclonal antibody raised against AGR2. It has been selected for its ability to recognize AGR2 in immunohistochemical staining and western blotting.

[ APPLICATIONS ]

Western blotting: 1:100-400

Immunocytochemistry in formalin fixed cells: 1:100-500

Immunohistochemistry in formalin fixed frozen section: 1:100-500

Immunohistochemistry in paraffin section: 1:50-200

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.

[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ STORAGE ]

Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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