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ACTR3 Polyclonal Antibody
ARP3
货号:YBAP11873 |
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规格: 50ul/100ul/200ul |
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价格: 980/1280/1890 |
Immunogen: | Recombinant protein of human ACTR3 | ||
Synonym: |
ARP3 | ||
Calculated MW: | 47kDa | ||
Observed MW: | Refer to figures | ||
Source: |
Rabbit |
Isotype: |
IgG | ||
Application: |
WB | ||
Purify: |
Affinity purification | ||
Concentration: |
1.89mg/ml | ||
Storage Buffer: |
PBS with 0.05% sodium azide, 50% glycerol, pH7.3 | ||
Storage: |
Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of k562, HL60, Hela, Jurkat, Cos1 and Cos7 cell, mouse spleen and liver tissue, using ACTR3 Polyclonal Antibody at dilution of 1:500 |
Protocols: |
Buffer-Formulation WB-Guide IHC-Guide IP-Guide IF-Guide |
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Background: |
Actin nucleation, the formation of new actin filaments from existing filaments, affects actin filament structure during cell motility, division, and intracellular trafficking. An important actin nucleation protein complex is the highly conserved ARP2/3 complex, consisting of ARP2, ARP3, and ARPC1-5. The ARP2/3 complex promotes branching of an existing actin filament and formation of a daughter filament following activation by nucleation-promoting factors, such as WASP/WAVE or cortactin. The formation of podosomes, small cellular projections that degrade the extracellular matrix, is enhanced by ARP2/3 complex action. ARP2/3 competes with caldesmon, an actin binding protein shown to negatively affect podosome formation). Along with N-WASP, the ARP2/3 complex regulates nuclear actin filament nucleation and controls actin polymerization during transcription.
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