

工作时间 :
周一~~周五
9:00 -18:00
在非工作时间,您可以通过邮件订购产品,订购时请写明详细联系方式,谢谢支持!
销售:18321282235
技术:021-60514606
传真:021-37680378
顾经理微信 扫一扫,关注我们
BTK Polyclonal Antibody
AGMX1; AT; ATK; BPK; IMD1; MGC126261; MGC126262; PSCTK1; XLA
货号:YBAP11948 |
|
![]() |
|
规格: 50ul/100ul/200ul |
|
价格: 980/1280/1890 |
Immunogen: | Recombinant protein of human BTK | ||
Synonym: |
AGMX1; AT; ATK; BPK; IMD1; MGC126261; MGC126262; PSCTK1; XLA | ||
Calculated MW: | 76kDa | ||
Observed MW: | Refer to figures | ||
Source: |
Rabbit |
Isotype: |
IgG | ||
Application: |
WB | ||
Purify: |
Affinity purification | ||
Concentration: |
2.43mg/ml | ||
Storage Buffer: |
PBS with 0.05% sodium azide, 50% glycerol, pH7.3 | ||
Storage: |
Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of Daudi cell, using BTK Polyclonal Antibody at dilution of 1:500 |
Protocols: |
Buffer-Formulation WB-Guide IHC-Guide IP-Guide IF-Guide |
||
Background: |
Bruton's tyrosine kinase (Btk) is a member of the Btk/Tec family of cytoplasmic tyrosine kinases. Like other Btk family members, it contains a pleckstrin homology (PH) domain and Src homology SH3 and SH2 domains. Btk plays an important role in B cell development. Activation of B cells by various ligands is accompanied by Btk membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane-localized Btk is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinases, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation. The activation of Btk is negatively regulated by PKCβ through phosphorylation of Btk at Ser180, which results in reduced membrane recruitment, transphosphorylation, and subsequent activation. The PKC inhibitory signal is likely to be a key determinant of the B cell receptor signaling threshold to maintain optimal Btk activity.
|
部门 |
姓名 | 手机 | |||
销售部 | 顾先生 | 1916510334@qq.com | 18321282235 | 1916510334 | |
技术部 | 技术支持 | 1781364813@qq.com | 13816899465 | 1781364813 |
全国免费电话:18321282235
销售: 18321282235
86-21-60514606
技术: 13816899465
传真: 021-37680378
The latest products