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SPIN2B Polyclonal Antibody
SPIN-2; dJ323P24.2; SPIN2_duplicate
货号:YBAP14658
价格: 980/1280/1890
Immunogen: Recombinant protein of human SPIN2B
Synonym:
SPIN-2; dJ323P24.2; SPIN2_duplicate
Calculated MW: 29kDa
Observed MW: Refer to figures
Source:
Rabbit
Isotype:
IgG
Reactivity: H;

Clonality: Polyclonal


Application:
ELISA WB IHC
Purify:
Affinity purification
Concentration:
1.1mg/ml
Storage Buffer:
PBS with 0.05% sodium azide, 50% glycerol, pH7.3
Storage:
Store at -20℃. Avoid freeze / thaw cycles.
SPIN2B Polyclonal Antibody


Western blot analysis of SP20 cell, using SPIN2B Polyclonal Antibody at dilution of 1:200

Protocols:
Buffer-Formulation WB-Guide IHC-Guide IP-Guide IF-Guide
Background:
Human SPIN2 expressed by 2 IL3-dependent murine myeloid cell lines was moderately antiapoptotic following IL3 removal, but it was unable to block apoptosis induced by the chemotherapy agent doxorubicin. On the contrary, SPIN2-expressing cells were more sensitive to doxorubicin-mediated cell death. Also, SPIN2 did not combat the proapoptotic effects of Fas ligand stimulation in the Jurkat human T-cell line. Deletion of the C-terminal amino acids of SPIN2 diminished its antiapoptotic activity. In the murine myeloid cells, SPIN2 overexpression increased the cell number doubling times and slowed their rate of growth. There was also an increased percentage of cells in G2/M, which was more pronounced following IL3 withdrawal.

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