YB80838Hu01 YB80838Hu01 YB80838Hu01

Otubain Otubain Otubain Otubain 1 (OTUB1) (OTUB1) (OTUB1) (OTUB1)

Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)

Instruction Instruction Instruction Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES

1th Edition (Revised in February, 2012)

[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: Otubain 1

Gene Names: OTUB1

Size: 100µg

Source: Recombinant

Expression Expression Expression Expression Host: E.coli

Function: Function: Function: Function: Hydrolase that can remove conjugated ubiquitin from proteins and plays an

important regulatory role at the level of protein turnover by preventing degradation.

Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered

unresponsive to antigen rechallenge and no longer respond to their cognate antigen.

Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy.

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm

Tissue Specificity: Specificity: Specificity: Specificity: Isoform 1 is ubiquitous. Isoform 2 is expressed only in lymphoid

tissues such as tonsils, lymph nodes, spleen and peripheral blood mononuclear cells.

[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]

Residues Residues Residues Residues: Met1~Lys271 (Accession # Q96FW1), with a N-terminal His-tag.

Grade & Purity: Purity: Purity: Purity: >97%, 32.53 kDa as determined by SDS-PAGE reducing conditions.

Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg(determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA;IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 32.53 kDa

[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in PBS.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Store at 4oC for short term storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term

storage. Avoid repeated freeze/thaw cycles.

Valid period: period: period: period: 12 months stored at -80oC.

[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]

The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which will

increase the chelating ability of the tag to Ni-Sepharose during purification.

MGHHHHHHSGS-MAAEEPQQQK QEPLGSDSEG VNCLAYDEAI MAQQDRIQQE IAVQNPLVSE RLELSVLYKE

YAEDDNIYQQ KIKDLHKKYS YIRKTRPDGN CFYRAFGFSH LEALLDDSKE LQRFKAVSAK SKEDLVSQGF

TEFTIEDFHN TFMDLIEQVE KQTSVADLLA SFNDQSTSDY LVVYLRLLTS GYLQRESKFF EHFIEGGRTV

KEFCQQEVEP MCKESDHIHI IALAQALSVS IQVEYMDRGE GGTTNPHIFP EGSEPKVYLL YRPGHYDILY K

[ REFERENCES REFERENCES REFERENCES REFERENCES ]

1. Borodovsky A., et al. (2002) Chem. Biol. 9:1149-1159. 2. Balakirev M.Y., et al. (2003) EMBO Rep. 4:517-522. 3. Stanisic V., et al. (2009) Borodovsky A., et al. J. Biol. Chem. 284:16135-16145.

4. Soares L., et al. (2004) Nat. Immunol. 5:45-54. 5. Edelmann M.J., et al. (2009) Biochem. J. 418:379-390.

6. Wang T., et al. (2009) J. Mol. Biol. 386:1011-1023.

 您可以点击 下载产品说明书, 或直接联系我们订购.